Boris Assyov
https://orcid.org/0000-0002-7365-2443; Fuat Bozok
https://orcid.org/0000-0002-9370-7712; Monica Slavova
https://orcid.org/0000-0003-4621-5173; Hatıra Taşkın
https://orcid.org/0000-0002-1784-4731
2 August 2023 ·
volume 45 · (
issue 8) · pp. 197–210 ·
PDF [full text]
Abstract: Entonaema is a peculiar and morphologically easily recognised fungal genus with disjunct and predominantly tropical-subtropical distribution. Entonaema cinnabarinum is typified on material from Australia and is the sole known species in Europe. It was first reported on this continent three decades ago, based on a collection from Bulgaria and remains so far one of the utmost rare European fungi with merely five sites known on the continent. After diligent search, the Bulgarian Entonaema was rediscovered in the area where it was first collected, and new sites were also found. Two ITS rDNA sequences of Bulgarian collections were obtained, appearing to be the first verified accessions of European origin. In the phylogenetic inference they appear closely related to an accession from South Korea, dubbed E. splendens. The outcomes of the phylogenetic analysis confirm the self-standing status of Entonaema, but its precise affiliation within the order Xylariales remains to be assessed further. Morphological characterisation, ample macroscopic and microscopic illustrations, as well as SEM images of ascospores of the new Bulgarian findings are included.
Keywords: Balkan mycota, biogeography, DNA-barcoding, Hypoxylaceae, taxonomy, xylarialean fungi
References [32]
Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990 Basic local alignment search tool. Journal of Molecular Biology 215 (3): 403–410.
https://doi.org/10.1016/S0022-2836(05)80360-2Anisimova M., Gascuel O. 2006 Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Systematic Biology 55 (4): 539–552.
https://doi.org/10.1080/10635150600755453Anisimova M., Gil M., Dufayard J.-F., Dessimoz C., Gascuel O. 2011 Survey of branch support methods demonstrates accuracy, power, and robustness of fast likelihood-based approximation schemes. Systematic Biology 60 (5): 685–699.
https://doi.org/10.1093/sysbio/syr041Assyov B. 2022 Barcoding and morphological characterization of Mycena cupressina (Agaricales, Fungi), a little-known and controversially treated member of section Supinae. Comptes rendus de l’Académie bulgare des Sciences 75 (11): 1595–1603.
https://doi.org/10.7546/CRABS.2022.11.06Bellanger J.-M., Moreau P.-A., Corriol G., Bidaud A., Chalange R., Dudova Z., Richard F. 2015 Plunging hands into the mushroom jar: a phylogenetic framework for Lyophyllaceae (Agaricales, Basidiomycota). Genetica 143: 169–194.
https://doi.org/10.1007/s10709-015-9823-8Benkert D. 1993 Kotlabaea macrospora Benkert sp. nov. und einige weitere bemerkenswerte Ascomyceten aus Bulgarien. Feddes Repertorium 104: 547–549.
https://doi.org/10.1002/fedr.19931040720Bozok F., Assyov B., Taskin H., Dogan H.H., Buyukalaca S. 2020 Molecular phylogenetic studies of Turkish boletes with emphasis on some recently described species. Nova Hedwigia 110 (1–2): 99–129.
https://doi.org/10.1127/nova_hedwigia/2019/0563Castresana J. 2000 Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17 (4): 540–552.
https://doi.org/10.1093/oxfordjournals.molbev.a026334Chevenet F., Brun C., Bañuls A.L., Jacq B., Christen R. 2006 TreeDyn: towards dynamic graphics and annotations for analyses of trees. BMC Bioinformatics 7: 439.
https://doi.org/10.1186/1471-2105-7-439Cooke M.C. 1887 Some Australian fungi. Grevillea 15 (76): 97–101.
Dereeper A., Guignon V., Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.-F., Guindon S., Lefort V., Lescot M., Claverie J.-M., Gascuel O. 2008 Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research 36 (Suppl. 2): 465–469.
https://doi.org/10.1093/nar/gkn180Edgar R.C. 2004 MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32 (5): 1792–1797.
https://doi.org/10.1093/nar/gkh340Fedosova A.G. 2012 New finging of Entonaema cinnabarinum (Xylariaceae, Ascomycota) in Europe. Vestnik Sankt-Peterburgskogo Universiteta Ser. 3 57 (1): 10–13.
Fournier J., Magni J.-F. 2004 Pyrenomycetes from southwestern France: Entonaema cinnabarinum (Cooke and Massee) Lloyd. pyrenomycetes.free.fr/entonaema/html/Entonaema_cinnabarina.htm
Fintha G., Benedek L., Orbán S. 2019 New macrofungial record in Hungary: Entonaema cinnabarinum (Cooke and Massee) Lloyd. Acta Biologica Plantarum Agriensis 7 (1): 127–130.
https://doi.org/10.21406/abpa.2019.7.127Guindon S., Gascuel O. 2003 A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52 (5): 696–704.
https://doi.org/10.1080/10635150390235520Hall T.A. 1999 BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hengstmengel J. 2020 Emendations to tissue typology in discomycetes. Mycological Progress 19 (6): 543–558.
https://doi.org/10.1007/s11557-020-01577-5Kim C.S., Jo J.W., Kwag Y.-N., Sung G.-H., Lee S.-G., Kim S.-Y., Shin C.-H., Han S.-K. 2015 Mushroom flora of Ulleung-gun and a newly recorded Bovista species in the Republic of Korea. Mycobiology 43 (3): 239–257.
https://doi.org/10.5941/MYCO.2015.43.3.239Kornerup A., Wanscher J.H. 1978 Methuen handbook of colour. Eyre Methuen, London, 252 pp.
Læssøe T. 1997 Entonaema cinnabarina – en eksotisk kernesvamp. Svampe 36: 21–22.
Pažoutová S., Follert S., Bitzer J., Keck M., Surup F., Šrůtka P., Holuša J., Stadler M. 2013 A new endophytic insect-associated Daldinia species, recognised from a comparison of secondary metabolite profiles and molecular phylogeny. Fungal Diversity 60: 107–123.
https://doi.org/10.1007/s13225-013-0238-5Rogers J.D. 1981 Sarcoxylon and Entonaema (Xylariaceae). Mycologia 73 (1): 28–61.
https://doi.org/10.1080/00275514.1981.12021319Rubio Domínguez E., Menédez Valderey J.L. 2011 Entonaema cinnabarina (Cooke and Massee) Lloyd. Revista Astanatura 334: 2.
Stadler M., Fournier J., Granmo A., Beltrán-Tejera E. 2008 The “red Hypoxylons” of the temperate and subtropical Northern hemisphere. North American Fungi 3 (7): 73–125.
https://doi.org/10.2509/naf2008.003.0075Stadler M., Fournier J., Læssøe T., Lechat C., Tichy H.V., Piepenbring M. 2008 Recognition of hypoxyloid and xylarioid Entonaema species and allied Xylaria species from a comparison of holomorphic morphology, HPLC profiles, and ribosomal DNA sequences. Mycological Progress 7: 53–73.
https://doi.org/10.1007/s11557-008-0553-5Stadler M., Lambert C., Wibberg D., Kalinowski J., Cox R.J., Kolařík M., Kuhnert E. 2020 Intragenomic polymorphisms in the ITS region of high-quality genomes of the Hypoxylaceae (Xylariales, Ascomycota). Mycological Progress 19: 235–245.
https://doi.org/10.1007/s11557-019-01552-9Stadler M., Læssøe T., Fournier J., Decock C., Schmieschek B., Tichy H.V., Peršoh D. 2014 A polyphasic taxonomy of Daldinia (Xylariaceae). Studies in Mycology 77: 1–143.
https://doi.org/10.3114/sim0016Stadler M., Yu-Ming J.U., Rogers J.D. 2004 Chemotaxonomy of Entonaema, Rhopalostroma and other Xylariaceae. Mycological Research 108 (3): 239–256.
https://doi.org/10.1017/S0953756204009347Šrůtka P., Pažoutová S., Kolařík M. 2007 Daldinia decipiens and Entonaema cinnabarina as fungal symbionts of Xiphydria wood wasps. ycological Research 111 (2): 224–231.
https://doi.org/10.1016/j.mycres.2006.10.006Triebel D., Peršoh D., Wollweber H., Stadler M. 2005 Phylogenetic relationships among Daldinia, Entonaema, and Hypoxylon as inferred from ITS nrDNA analyses of Xylariales. Nova Hedwigia 80 (1–2): 25–43.
https://doi.org/10.1127/0029-5035/2005/0080-0025Wibberg D., Stadler M., Lambert C., Bunk B., Spröer C., Rückert C., Kalinowski J., Cox R.J., Kuhnert E. 2021 High quality genome sequences of thirteen Hypoxylaceae (Ascomycota) strengthen the phylogenetic family backbone and enable the discovery of new taxa. Fungal Diversity 106: 7–28.
https://doi.org/10.1007/s13225-020-00447-5