New and rare fungus gnats for the fauna of Bulgaria (Diptera: Nematocera: Mycetophilidae)

The paper provides information about two newly recorded species for the fauna of Bulgaria: Docosia rohaceki Sevcik, 2006 and Leia graeca Bechev, 1997 and new localities for another two rare species in Bulgaria. Azana (A.) bulga­ rense Coher, 1995 is proposed as a synonym of Azana (A.) flavohalterata Strobl, 1909.


Introduction
So far, 319 species of fungus gnats are known from the territory of Bulgaria (Bechev, 2006(Bechev, , 2010Bechev & Pavlova, 2012, 2016Kurina & Chandler, 2018;Pavlova, 2020;Pavlova & Stojanova (in press)). As a result of our study, here we present the first records of two species in Bulgaria and an update on the distribution of two species that have been previously recorded in the country only from single localities.

Material and methods
Part of the material was collected within the framework of the project "Studies on biodiversity of model animal groups in Kermes Oak communities (Quercus coccifera L.), anthropogenic impact assessment and proposing protection measures" (Bulgarian National Science Fund No B-MU-1106/01) (Langourov & Simov, 2006). The rest of the material is gathered by the author. Three sampling techniques were used: traps, tree traps -white coloured modified Moericke traps (Langourov, 2001;Langourov & Simov, 2006), both filled with formalin or propylene glycol, and Townes type Malaise trap. The traps were changed each month. The chosen localities are in critically endangered Mediterranean habitats in Bulgaria (Figs 1-2): shrubs and low woods of the Kermes oak (Quercus coccifera) Langourov et al., 2012) and forests of Grecian juniper (Juniperus excelsa) (Tzonev & Dimitrov, 2015).
The material is deposited in the author's collection and in the collection of the National Museum of Natural History, Sofia.

Results and discussion
List of records    New species for the fauna of the Balkan Peninsula. Previously, it has been known only from Slovakia (Ševčík, 2006). This is the most south-eastern record for the species. Photo of male genitalia of the studied specimens is presented on figure 3. Bechev, 1988  The species is described from Bulgaria, Western Balkan Range, Vrachanska Planina, Ledenika (Bechev, 1988). Until now, it has been known only from its type locality and another one in Greece (Kurina, 2004). Balkan endemic. Based on the present observations, a wider range of the species could be expected. Bechev, 1997    This is the first record of the species in Bulgaria. The species is known up to now with several localities from Greece (Bechev, 1997;Chandler et al., 2005). Balkan endemic species. Photo of lateral view of the male genitalia of the Bulgarian specimen is presented on figure 4. Unfortunately, part of gonostyle is a bit broken but it is still visible that is forked.

Subfamily Sciophilinae
Azana ( Widespread in the Mediterranean region, recorded in Spain, Cyprus, Greece (Thessaly, Crete, Samos, Paxos, Milos) and Israel (Chandler, 1994;Chandler & Ribeiro, 1995;Chandler & Blasco-Zumeta, 2001;Kurina, 2004). In Bulgaria, until now it is reported from a single locality -the type locality of A. bulga rense (Strandzha Mts). The unique male is described as a new species under the name Azana (Azana) bul garense by Coher (1995). According to Chandler & Blasco-Zumeta (2001), the genitalia that Coher (1995) had figured under the name bulgarense seems identical with Azana (A.) flavohalterata. We found no structural difference in the male genitalia from typical Azana (A.) flavohalterata has been found (plate 2 in Coher, 1995;fig. 48 in Chandler & Blasco-Zumeta, 2001 and Fig. 5). Therefore, we concluded them to be conspecific and we proposed a new synonymy. The female specimens were associated with the male ones.